WebJan 20, 2024 · We have sought to synthesize immunogenicity-free, short dsRNA-containing RNA circles as PKR inhibitors. Here, we report that RNA circles synthesized by permuted self-splicing thymidylate synthase (td) introns from T4 bacteriophage or by Anabaena pre-tRNA group I intron could induce an immune response. Autocatalytic splicing introduces … WebThe aim of this study was to identify circRNAs involved in lung adenocarcinoma (LUAD) using bioinformatics analysis. Methods: CircRNA (GSE101684, GSE101586), miRNA (GSE135918), and mRNA (GSE130779) microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database to identify differentially expressed circRNAs …
Sensing Self and Foreign Circular RNAs by Intron Identity
WebFour-week-old male BALB/c nude mice were divided into 2 groups (n=5 for each group). Then, HCT116 cells (hsa_circRNA_102958 silencing or control) were subcutaneously inoculated into the right flank of each nude mouse. Every 1 week, the tumor volumes were measured by the formula: Volume = 1/2 (length × width 2). This assay was approved by … WebJan 6, 2024 · PIE transcription templates commonly utilized for in vitro circRNA synthesis employ group I intron sequences from either the Anabaena tRNA Leu gene or the … phillip bird norman
CircRNAs: a new target for the diagnosis and treatment of ... - Nature
WebApr 25, 2024 · Conn et al. further demonstrates that the disruption of splicing factor QKI or its binding sites on flanking introns attenuates circRNA formation during EMT, and Errichelli et al. find that disruption of splicing factor FUS in mouse motor neurons affects circRNA expression . Our group also show that splicing factor ESRP1 can promote circRNA ... WebJul 20, 2024 · CircRNA activation of innate immunity is independent of a 5′ triphosphate, double-stranded RNA structure, or the primary sequence of the foreign circRNA. Instead, self-nonself discrimination depends on the intron that programs the circRNA. ... The group I intron of phage T4 thymidylate synthase (td) gene is well characterized to circularize ... WebJan 15, 2024 · WST-8 assay. Cells at a density of 5.0 × 10 4 cells/mL were plated in a 96-well plate and divided into five groups: miR-21-5p group (miR-2-5p over-expression), NC group (infected with negative control lentiviral vector), control group (no infection), sh-Tiam1 group (infected with lentiviral vector expressing short hairpin (sh)-Tiam1), and sh … phillip bishop