D3922 thermo fisher scientific

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WebFeature papers are submitted upon individual invitation or recommendation by the scientific editors and must receive positive feedback from the reviewers. ... Alternatively, for BODIPY staining, 3D spheroids in 6 super-low-attachment well dishes were incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 1 h ... WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …

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WebJul 4, 2024 · They were cultured in Dulbecco’s Modified Eagle’s medium (Thermo Fisher Scientific) media containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) in an incubator at 28℃. ... (D3922; Thermo Fisher Scientific) for 30 min, followed by thrice PBS washes. The IECs were observed using a laser scanning confocal microscope … joe thomas cleveland browns twitter

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WebMay 14, 2024 · Most recently, to understand the molecular etiology of thyroid-associated orbitopathy, our group utilized the 3D cell culture of human orbital fibroblasts, and found that hypoxia-inducible factor... WebDec 12, 2024 · Lipophilic green-fluorescent BODIPY 493/503 (D3922, Thermo Fisher Scientific, Waltham, MA, USA) was used for lipid staining. SkMs were seeded at a density of 1000 cells/cm 2 and treated as indicated. WebMeasurement Range: -50 to +392°F/-45 to +200°C NSF® Certified One-button operation 3 mm tip 5"/12.7 cm stem Shatterproof Food-safe ABS plastic with BioCote® Stainless … integrity insurance agency inc

BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4 …

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Web货号: D3922 相关应用: Cellular Imaging 技术支持客户服务收藏夹产品概述推荐产品文件常见问题解答引用和文献 BODIPY™ 493/503 具有非极性结构、吸收波长较长且发荧 … WebJan 1, 2024 · Shikonin was purchased from Selleck (S8279). Lipofectamine 3000 for siRNA transfection (Invitrogen), HilyMax for plasmid transfection (Dojindo, Kumamoto, Japan). Puromycin（Sigma，St. Louis, MO, USA). BODIPY 493/503 … integrity insurance agency punta gordaWebIECs were cultured in 12-well plates for 36 h, then washed twice with PBS and incubated with the 5 g mL −1 Bodipy 493/503 (D3922; Thermo Fisher Scientific, Waltham, MA, USA) for 30 min, followed by three PBS washes. A laser scanning confocal microscope (Leica DMI8) was used to visualize the fluorescence intensity in the IECs. integrity insurance agency llc

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D3922 thermo fisher scientific

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WebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS. WebJan 9, 2024 · The BODIPY 493/503 (D3922; Thermo Fisher Scientific, USA) staining was performed for primary IECs, as described by Wei et al. . The laser scanning confocal microscope (Leica Microsystems, Germany) …

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WebSU24 - Scientific research and development Uses advised againstNot for consumer use. Details of the supplier of the safety data sheet Manufacturer/Supplier 24 hour Emergency … WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …

WebNov 8, 2024 · The cells were co-stained with BODIPY™ 493/503 for lipid droplets (D3922; Thermo Fisher Scientific), LysoTracker™ Deep Red (L12492; Thermo Fisher Scientific), or MitoTracker™ (M7510; Thermo Fisher Scientific). Nuclei were blue-stained with 4′,6-diamidino-2-phenylindole (DAPI). Tumor xenograft. All animal study procedures were … WebBioactive Small Molecules D3922 Print Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) …

WebFeb 8, 2013 · This Thermo Scientific full-size Ultrapure mixed bed ion exchange hose nipple cartridge is an economical way to purify water volumes up to 75L/hour. It is constructed of 100% virgin polypropylene cartridge with superior quality resin. The color change indicator identifies when resin is exhausted. It is designed for non-pressurized … WebInvitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) Catalog number: D3922 Related applications: Cellular Imaging Technical …

WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, …

WebFeb 9, 2024 · To visualize the accumulation of lipids in response to OA treatment, we used boron-dipyrromethene (BODIPY) 493/503 (D3922, Thermo Fisher Scientific, MA, USA), which labels specifically ... joe thomas current weightWebNov 18, 2024 · DFCP1 is an ER-associated protein with two PI (3)P-binding FYVE domains. In WT cells, myc-DFCP1 formed very small punctate structures ( Figure 1E) ( Axe et al., 2008; Li et al., 2024 ). In SKO cells, the myc-DFCP1 structures appeared highly irregular and significantly enlarged ( Figure 1, E and F). integrity insurance agency idahoWebAug 1, 2024 · For BODIPY lipid staining [6–8], 4% paraformaldehyde fixed 3D sphenoids were incubated in 0.1% BODIPY (# D3922, Thermo Fisher Scientific), 0.1% DAPI (#D523, Doujin, Japan) and 0.1% phalloidin (#20,553, Funakoshi, Japan) in phosphate buffered saline containing 3% bovine serum albumin for 3 hrs. joe thomas coachingWebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in PBS for 1 hr, and then fixed in 4% paraformaldehyde (PFA) in PBS for 10 min at room temperature. integrity insurance agency sealy txWebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … integrity insurance agency houston txWebFeb 1, 2024 · We performed ORO and Bodipy 493/503 (D3922; Thermo Fisher Scientific, Waltham, MA, USA) staining based on the protocols described in our previous research [4, 13]. The ten fields were selected from each sample to qualify relative areas of LDs in ORO by Image J. We used the laser scanning confocal microscope (Leica Microsystems, … joe thomas englishWebJan 16, 2024 · Briefly, 2D or 3D 3T3-L1 cells were incubated in a mixture of 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor 594 phalloidin (# 20553, Funakoshi, Tokyo, Japan), and DAPI (# D523, Dojindo, Kumamoto, Japan), at 1:1000 dilutions in PBS for 1 hr, and thereafter fixed in 4% paraformaldehyde in PBS for … joe thomas fishing show