F4/80 antibody alexa

Author: delj

August undefined, 2024

WebThe T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). F4/80 … WebTo characterize the cellular localization of MIC3, cells were incubated with primary anti-MIC3 mouse monoclonal antibody (Anti-MIC3) and PE-conjugated antibody against mouse F4/80 overnight at 4°C. After being washed, cells were incubated with secondary Alexa Fluor 488 AffiniPure goat anti-mouse IgG antibody (Anti-IgG).

Anti-F4-80 Antibody Products Biocompare

WebProduct Description. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye under optimal conditions and tested in-house for direct … WebF4/80 is a well-characterized and extensively referenced membrane protein and is the best known mature mouse macrophage and microglial marker. The anti-F4/80 antibody clone CI:A3-1 is the original monoclonal produced against the F4/80 antigen in Siamon Gordon’s laboratory at the University of Oxford (Gordon and Austyn, 1981). Today, Bio-Rad ... townsville nab

F4/80 Antibodies Bio-Rad

WebF4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a … WebThese antibodies target F4/80 in Mouse, Human and Rat samples. Our F4/80 monoclonal and polyclonal antibodies are developed in Rat and Rabbit. These antibodies have been verified by Relative expression and Cell treatment to confirm specificity to F4/80. Find the F4/80 antibody that fits your needs. WebImmunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-F4/80 antibody [F4/80] (ab204467) ab204467 staining F4/80 in Raw264.7 cells. The cells were fixed … townsville naidoc

Alexa Fluor™ 488 Rat Anti-Mouse F4/80 - BD Biosciences

F4/80 Monoclonal Antibody (BM8), Alexa Fluor™ 488 (53 …

WebStaining of mouse resident peritoneal exudate cells with Anti-Mouse CD11b APC (Product # 17-0112-82) and 0.25 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.25 µg of Anti-Mouse … WebF4/80 Monoclonal Antibody (BM8), Alexa Fluor 700, eBioscience™ ... The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this … townsville native nurseryWebAfter 4 h of incubation, anti-F4/80-APC antibody was used to label BMDMs, and these cells were collected for flow cytometry analysis. ... For CLSM observation of CRT exposure and HMGB1 release, the Alexa Fluor 488 anti-Calericulin antibody (Abcam, USA) and Alexa Fluor 594 anti-HMGB1 antibody (Abcam, USA) were used to stain the treated B16-F10 ... townsville national parks

"WebC57BL/6 mouse resident peritoneal macrophages were stained with CD11b Monoclonal Antibody, eFluor 450 (Product # 48-0112-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 561 (Product # 505-4321 … " - F4/80 antibody alexa

F4/80 antibody alexa

F4/80 Monoclonal Antibody (BM8), Alexa Fluor 488, …

WebThe BM8 antibody reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. F4/80 is considered a marker of choice for the identification of mature tissue macrophages, being broadly but variably expressed by this cell type ...

Did you know?

WebTarget Information. F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall … WebThe molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1). Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2).

WebTarget Information. F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human … WebTwo hours after injection, peritoneal exudates were collected and stained with an FITC-conjugated anti-F4/80 antibody and analyzed by flow cytometry. TAMRA positive and F4/80 negative cells were considered as unengulfed apoptotic cells which were counted using Sphero AccuCount particles (ACBP-50-10, Spherotech, Lake Forest, IL, USA).

WebAlexa Fluor 350 (4) Alexa Fluor 405 (4) Alexa Fluor 488 (4)----- All A - Z -----Alexa Fluor 350 (4 ) Alexa Fluor ... Mouse, C. elegans, and E. coli. Each F4/80 antibody is fully … WebProduct Description. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye under optimal conditions and tested in-house for direct immunofluorescent analysis in mouse tissue. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated F4/80 (D4C8V) XP ® Rabbit mAb ...

WebF4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal … townsville nafaWebF4/80 is a well-characterized and extensively referenced membrane protein and is the best known mature mouse macrophage and microglial marker. The anti-F4/80 antibody clone CI:A3-1 is the original monoclonal produced against the F4/80 antigen in Siamon Gordon’s laboratory at the University of Oxford (Gordon and Austyn, 1981). townsville napaWebMouse F4/80 Alexa Fluor® 350-conjugated Antibody . FAB5580U. 1 Image. Flow. Show Size & Price. Mouse F4/80 Alexa Fluor® 405-conjugated Antibody . FAB5580V. 1 Image. Flow. Show Size & Price. page of 1. Back to Search Results. Apply. FiltersClear All. Category Clear. Primary Antibodies (10) cDNA Clones (1) townsville native titleWebThe section was costained with anti-mouse F4/80 Antibody (clone BM8) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse I-A/I-E Antibody (clone M5/114.15.2) Alexa Fluor® 594 … townsville nbl1WebSep 23, 2024 · F4/80 Antibody (C-7) is available as both the non-conjugated anti-F4/80 antibody form, as well as multiple conjugated forms of anti-F4/80 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor ® conjugates. The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, … townsville nc countyWebF4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and ... townsville nbl teamWebThe T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). F4/80 is a 160 kDa glycoprotein that belongs to the EGF-TM7 family of seven-transmembrane spanning cell surface molecules. It is expressed on the surface of granulocytes and a … townsville natural landmarks