WebThis supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ™ SYBR® Green supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude.
iScript™cDNA Synthesis Kit
WebWith PowerUp SYBR Green Master Mix, we’ve taken the best of EXPRESS SYBR GreenER SuperMix and added additional capabilities for your gene expression analysis. EXPRESS SYBR GreenER qPCR SuperMix kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. WebJun 28, 2016 · RNA (2 µg) was used for first-strand cDNA synthesis with reverse transcriptase (Promega Corporation, Madison, WI, USA), according to the manufacturer's protocol. cDNA was amplified by qPCR in a CFX96 Touch Real-Time PCR Detection system with CFX Manager 3.0 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) using iQ … jerry atric samurai pizza cats
PerfeCTa SYBR Green SuperMix - Quantabio
WebAug 10, 2011 · The iQ™ SYBR ® Green Supermix contains 3 mM MgCl 2. 5'-nuclease probe hydrolysis reactions consisted of 0.4 U iTaq DNA polymerase (BIO-RAD, Hercules, CA), 1 μL 10X iTaq™ buffer (BIO-RAD, Hercules, CA), 3 mM MgCl 2, 800 μM dNTPs, and 200 nM each of the forward and reverse primers and the probe, 20 to 2 × 10 6 copies of the synthetic … WebAug 25, 2015 · Real-time PCR was performed using the iQ SYBR Green Supermix kit (Bio-Rad), with the following temperature cycles: cycle 1 pre-denaturation (10 min, 95 °C), cycle 2 repeated 50 times: denaturation (30 s, 95 °C), annealing (30 s at the annealing temperatures— T a, indicated in Table 2), and elongation (45 s, 72 °C) steps and cycle 3 … Web(3) Preventing erosion of X-chromosome inactivation in human embryonic stem cells Nature Communications December 1, 2024 Marissa Cloutier et al. (#233-FB) was obtained from R&D Systems; SsoAdvance SYBR Green Supermix was obtained from Bio-Rad (#1725272); 3 M Sodium Acetate was obtained from Teknova (#S0298); Triton X-100 was obtained from ... lamborghini huracan manual gearbox