Mcherry gal9
WebAddgene: A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery. Browse Alan Sabirsh Munson et al A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery. Web18 jun. 2015 · In KRAS mut CRC, rLGALS9 acts as a lysosomal inhibitor that inhibits autophagosome-lysosome fusion, leading to autophagosome accumulation, excessive lysosomal swelling and cell death. This antitumor activity of rLGALS9 directly correlates with elevated basal autophagic flux in KRAS mut cancer cells.
Mcherry gal9
Did you know?
WebSequences mCherry-GAL9 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Analyze Sequence GenBank SnapGene File Help Web16 feb. 2024 · A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery. 1 Please help EMBL-EBI keep …
WebGalectin-9 (GAL9) is a known marker of endosomal membrane disruption and can be utilized to quantitate endosomal rupture 22 . The initially selected 7 compounds and all …
WebGalectin-9 (GAL9) is a known marker of endosomal membrane disruption and can be utilized to quantitate endosomal rupture 22 . The initially selected 7 compounds and all … Web16 feb. 2024 · mCherry-GAL9 responds to polymeric nanoparticles. a Overview of polymer names and compositions. Polymers are comprised of DEAEMA (60%) and BMA (40%) at …
WebmCherry (N terminal on backbone) Cloning Information Cloning method Restriction Enzyme 5′ cloning site HindIII (not destroyed) 3′ cloning site EcoRI (not destroyed) 5′ sequencing …
WebCell expression of the mCherry-GAL9 protein was verified by western blotting using an α-mCherry antibody for detection (Fig. 1d). Two prominent bands were observed, corresponding to full-length (~70kDa) and truncated (~50kDa) mCherry-GAL9 (consistent with the loss of GAL9’sC- terminal CRD (~20kDa) due to protease cleavage26). portable memory card storageWebDownload scientific diagram Use of mCherry-GAL9 reporter line with automated particle formulation a Overview of experimental approach. particles are formed by automated … portable metal building kitsWebmCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very … irs approved refineriesWebGalectin-9 (GAL9) is a known marker of endosomal membrane disruption and can be utilized to quantitate endosomal rupture 22 . The initially selected 7 compounds and all … irs approved public charitiesWebGal9 punctate (orange) reported as a percentage of max quantitation, normalized to nuclei signal. Cell survival (gray) reported as a percentage of all cells normalized to a DMSO … portable mesh for fishingWebCy5-mRNA mCherry-GAL9 EGFP Control +Rapamycin +KU0063794 g +Torin1 Supplementary Fig. 6 – mTOR inhibitors reduce LNP cargo protein translation a Representative fluorescence images of Huh7 mCherry-GAL9 cells dosed with 0.5 µg/ml MC3 LNPs and vehicle, Rapamycin (0.1 µM) or KU0063794 (0.1 µM) for 12h. Scale bar … irs approved software vendorsWebSequences mCherry-GAL9 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated … portable metal bleachers